WO2024256996 - C2 CARBON SOURCE-RESPONSIVE PROMOTERS
National phase entry:
Publication Number
WO/2024/256996
Publication Date
19.12.2024
International Application No.
PCT/IB2024/055767
International Filing Date
12.06.2024
Title **
[English]
C2 CARBON SOURCE-RESPONSIVE PROMOTERS
[French]
PROMOTEURS SENSIBLES A LA SOURCE DE CARBONE C2
Applicants **
DANSTAR FERMENT AG
Poststrasse 30
CH-6300 Zug, CH
Inventors
PANAITIU, Alexandra-Elena
c/o Mascoma LLC
67 Etna Road #200
Lebanon, New Hampshire 03766, US
CALCINES-CRUZ, Carlos
c/o Mascoma LLC
67 Etna Road #200
Lebanon, New Hampshire 03776, US
PRIVETT, Britney Rose
c/o Mascoma LLC
67 Etna Road #200
Lebanon, New Hampshire 03776, US
ARGYROS, David Aaron
c/o Mascoma LLC
67 Etna Road #200
Lebanon, New Hampshire 03766, US
Priority Data
63/508,378
15.06.2023
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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International Searching Authority |
EPO
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| Applicant's Legal Status |
Legal Entity
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| * | |
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| * | |
| * | |
| Entry into National Phase under |
Chapter I
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| Translation |
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Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 2948 | |
| EPO | Filing, Examination | 16092 | |
| Japan | Filing | 597 | |
| South Korea | Filing | 575 | |
| USA | Filing, Examination | 10010 |
Total: 30222 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
The present disclosure concerns promoters engineered to increase their responsiveness to a C2 carbon source. The engineered promoters include at least one external carbon source-responsive element (CSRE) located upstream and proximal to the transcription start site of a gene operatively associated thereto. The engineered promoters can be used in a heterologous nucleic acid molecule, a vector, or an expression cassette to promote the expression of a gene in a microbe. The present disclosure also concerns a method for generating the engineered promoters as well as a method for expressing a gene in a recombination microbial host cell using the engineered promoter(s).[French]
La présente invention concerne des promoteurs modifiés pour augmenter leur réactivité à une source de carbone C2. Les promoteurs modifiés comprennent au moins un élément sensible à la source de carbone externe (CSRE) situé en amont et à proximité du site de début de transcription d'un gène associé de manière fonctionnelle à celui-ci. Les promoteurs modifiés peuvent être utilisés dans une molécule d'acide nucléique hétérologue, un vecteur ou une cassette d'expression pour favoriser l'expression d'un gène dans un microbe. La présente invention concerne également un procédé de génération des promoteurs modifiés ainsi qu'un procédé d'expression d'un gène dans une cellule hôte microbienne de recombinaison à l'aide du ou des promoteurs modifiés.