WO2024161325 - SYSTEMS AND METHODS FOR IMPROVED SEQUENCE COVERAGE IN ANALYSIS OF LARGE POLYPEPTIDES
National phase entry is expected:
Publication Number
WO/2024/161325
Publication Date
08.08.2024
International Application No.
PCT/IB2024/050891
International Filing Date
31.01.2024
Title **
[English]
SYSTEMS AND METHODS FOR IMPROVED SEQUENCE COVERAGE IN ANALYSIS OF LARGE POLYPEPTIDES
[French]
SYSTÈMES ET PROCÉDÉS POUR UNE COUVERTURE DE SÉQUENCE AMÉLIORÉE DANS L'ANALYSE DE GROS POLYPEPTIDES
Applicants **
DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
33 Marsiling Industrial Estate Road 3
#04-06
Singapore 739256, SG
Inventors
JIN, Wen
c/o Sciex
71 Four Valley Drive
Concord, Ontario L4K 4V8, CA
LIU, Haichuan
c/o Sciex
1201 Radio Road
Redwood City, California 94065, US
RYUMIN, Pavel
c/o Sciex
71 Four Valley Drive
Concord, Ontario L4K 4V8, CA
ZHANG, Yuzhuo
c/o Sciex
1201 Radio Road
Redwood City, California 94065, US
Priority Data
63/442,645
01.02.2023
US
63/466,880
16.05.2023
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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| * | |
International Searching Authority |
EPO
* |
| Applicant's Legal Status |
Legal Entity
* |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Translation |
|
Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
** IP-Coster compiles data from publicly available sources. If this data includes your personal information, you can contact us to request its removal.
Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1937 | |
| EPO | Filing, Examination | 12419 | |
| Japan | Filing | 588 | |
| South Korea | Filing | 606 | |
| USA | Filing, Examination | 7510 |
Total: 23060 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
In some embodiments, a method for improved sequence coverage of a polypeptide comprises removing interchain disulfide bonds and retaining essentially all intrachain disulfide bonds of a first sample and performing a first mass spectrometry (MS) analysis of the first sample to determine a first amino acid sequence, removing interchain disulfide bonds and intrachain disulfide bonds of a second sample and performing a second MS analysis to determine a second amino acid sequence, and combining the first and second sequence to determine a combined amino acid sequence of the polypeptide.[French]
Dans certains modes de réalisation, un procédé de couverture de séquence améliorée d'un polypeptide consiste en l'élimination de ponts disulfure inter-chaînes et la conservation essentiellement de tous les ponts disulfure intra-chaînes d'un premier échantillon, la réalisation d'une première analyse par spectrométrie de masse (MS) du premier échantillon pour déterminer une première séquence d'acides aminés, l'élimination de ponts disulfure inter-chaînes et de ponts disulfure intra-chaînes d'un second échantillon et la réalisation d'une seconde analyse MS pour déterminer une seconde séquence d'acides aminés, et la combinaison des première et seconde séquences pour déterminer une séquence d'acides aminés combinée du polypeptide.