WO2024141901 - HEAT-BASED TRANSFER OF REACTION PRODUCTS MADE IN SITU TO A PLANAR SUPPORT
National phase entry:
Publication Number
WO/2024/141901
Publication Date
04.07.2024
International Application No.
PCT/IB2023/063140
International Filing Date
21.12.2023
Title **
[English]
HEAT-BASED TRANSFER OF REACTION PRODUCTS MADE IN SITU TO A PLANAR SUPPORT
[French]
TRANSFERT À CHAUD DE PRODUITS DE RÉACTION FABRIQUÉS IN SITU SUR UN SUPPORT PLAN
Applicants **
MOLECULENT AB
Neglinge Udde 4
133 34 Saltsjöbaden, SE
Inventors
ERICSSON, Olof John
Neglinge Udde 4
133 34 Saltsjöbaden, SE
Priority Data
63/435,790
28.12.2022
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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International Searching Authority |
EPO
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| Applicant's Legal Status |
Legal Entity
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| * | |
| * | |
| * | |
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| Entry into National Phase under |
Chapter I
* |
| Translation |
|
Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1919 | |
| EPO | Filing, Examination | 7785 | |
| Japan | Filing | 587 | |
| South Korea | Filing | 574 | |
| USA | Filing, Examination | 3160 |
Total: 14025 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Provided herein, among other things, is method for analyzing a sample. In some embodiments, the method may comprise: contacting a planar biological sample with an exogenous oligonucleotide or a conjugate comprising antibody and oligonucleotide under conditions by which the exogenous oligonucleotide or conjugate specifically binds to sites in or on the sample, performing one or more steps to cleave and/or extend, e.g. by primer extension or ligation, the exogenous oligonucleotide or complement thereof in situ, placing the sample in contact with a planar support; and incubating the sample and planar support at an elevated temperature, thereby transferring the products to the planar support in a way that preserves the spatial relationship of the products in the sample.[French]
L'invention concerne, entre autres, un procédé d'analyse d'un échantillon. Selon certains modes de réalisation, le procédé peut comprendre les étapes suivantes : mise en contact d'un échantillon biologique plan avec un oligonucléotide exogène ou un conjugué comprenant un anticorps et un oligonucléotide dans des conditions garantissant que l'oligonucléotide exogène ou le conjugué se lie spécifiquement à des sites dans ou sur l'échantillon ; réalisation d'une ou plusieurs étapes visant à cliver et/ou à allonger, par ex. par extension d'amorce ou ligature, l'oligonucléotide exogène ou son complément in situ, en mettant l'échantillon en contact avec un support plan ; et incubation de l'échantillon et du support plan à une température élevée, transférant ainsi les produits sur le support plan d'une manière qui préserve la relation spatiale des produits dans l'échantillon.