WO2023112000 - AN IMPROVED METHOD FOR SEPARATION OF LOW MOLECULAR WEIGHT PROTEINS
National phase entry:
Publication Number
WO/2023/112000
Publication Date
22.06.2023
International Application No.
PCT/IB2022/062426
International Filing Date
17.12.2022
Title **
[English]
AN IMPROVED METHOD FOR SEPARATION OF LOW MOLECULAR WEIGHT PROTEINS
[French]
PROCÉDÉ AMÉLIORÉ DE SÉPARATION DE PROTÉINES DE FAIBLE POIDS MOLÉCULAIRE
Applicants **
KASHIV BIOSCIENCES, LLC
Inventors
UPADHYAY, Roshan Ganeshlal
KARDANI, Jaykumar Rameshchandra
SHAH, Aakansha
Priority Data
202121058987
17.12.2021
IN
Application details
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International Searching Authority |
USPTO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2014 | |
| EPO | Filing, Examination, Granting | 11558 | |
| Japan | Filing, Examination, Granting | 2061 | |
| South Korea | Filing, Examination, Granting | 1937 | |
| USA | Filing, Examination, Granting | 4310 |
Total:
21,880
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The present invention provides the method for the separation of at least more than two Low molecular weight (LMWs) impurities present in the protein mixture comprising fusion protein CTL4-IgG1 by using Capillary Electrophoresis-Sodium dodecyl sulfate (CE-SDS) wherein the loading amount of protein mixture is more than 270 µg. The invention further provides a ratio of protein to sodium dodecyl sulfate is 2.7:1 or higher.[French]
La présente invention concerne le procédé de séparation d'au moins plus de deux impuretés de faible poids moléculaire (LMW) présentes dans le mélange de protéines comprenant la protéine de fusion CTL4-IgG1 en utilisant du dodécylsulfate de sodium d'électrophorèse capillaire (CE-SDS), la quantité de charge du mélange de protéines étant supérieure à 270 µg. L'invention concerne en outre un rapport entre la protéine et le dodécylsulfate de sodium supérieur ou égal à 2,7 : 1.