WO2023031793 - BIOCOMPATIBLE UNDERLAYS FOR LIVING EXTRACTION OF HYDROCARBONS FROM ENGINEERED MICROBES
National phase entry:
Publication Number
WO/2023/031793
Publication Date
09.03.2023
International Application No.
PCT/IB2022/058125
International Filing Date
30.08.2022
Title **
[English]
BIOCOMPATIBLE UNDERLAYS FOR LIVING EXTRACTION OF HYDROCARBONS FROM ENGINEERED MICROBES
[French]
SOUS-COUCHES BIOCOMPATIBLES POUR EXTRACTION VIVANTE D'HYDROCARBURES À PARTIR DE MICROBES MODIFIÉS
Applicants **
KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
Technology Transfer Office
4700 King Abdullah University of Science and Technology
Thuwal, 23955-6900, SA
Inventors
LAUERSEN, Kyle Jonathan
Technology Transfer Office
4700 King Abdullah University of Science and Technology
Thuwal, 23955-6900, SA
OVERMANS, Sebastian
Technology Transfer Office
4700 King Abdullah University of Science and Technology
Thuwal, 23955-6900, SA
Priority Data
63/239,142
31.08.2021
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
| * | |
| * | |
International Searching Authority |
EPO
* |
| Applicant's Legal Status |
Legal Entity
* |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Translation |
|
Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
** IP-Coster compiles data from publicly available sources. If this data includes your personal information, you can contact us to request its removal.
Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1738 | |
| EPO | Filing, Examination | 9628 | |
| Japan | Filing | 594 | |
| South Korea | Filing | 575 | |
| USA | Filing, Examination | 4310 |
Total: 16845 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Methods and systems that use fluorocarbons for designing scalable living, in-line extraction of heterologous metabolites from microbial cultures are disclosed. The disclosed systems and methods are organism agnostic and be applied to bacteria, yeasts, filamentous microbes, and algae. The disclosed methods and systems use pperfluorocarbon solvent to extract terpenes from engineered microorganisms and allows non-lethal milking of hydrocarbon products from filamentous and single-celled/multi-celled microbial cultures. The disclosed process preferably does not use pressure gradients and relies on the physical interaction of microbes with fluorocarbon at satp and standard temperature of microbial cultivation to partition non-native products. The disclosed process and system also allow for increased production of products providing a physical sink which enables the forward reactions to produce more products than in its absence.[French]
L'invention concerne des procédés et des systèmes utilisant des fluorocarbones pour concevoir une extraction en ligne vivante évolutive de métabolites hétérologues à partir de cultures microbiennes. Les systèmes et les procédés de l'invention sont agnostiques de l'organisme et peuvent être appliqués à des bactéries, des levures, des microbes filamenteux et des algues. Les procédés et systèmes divulgués utilisent un solvant à base de perfluorocarbone pour extraire les terpènes des micro-organismes modifiés et permettent une traite non létale des produits hydrocarbonés des cultures microbiennes filamenteuses et unicellulaires/multicellulaires. Le procédé divulgué n'utilise de préférence pas de gradients de pression et s'appuie sur l'interaction physique des microbes avec le fluorocarbone au satp et à la température standard de la culture microbienne pour séparer les produits non natifs. Le procédé et le système divulgués permettent également une production accrue de produits en fournissant un puits physique qui permet aux réactions en avant de produire plus de produits qu'en son absence.