WO2023099972 - AN IMMUNOFLUORESCENCE DETECTING EQUIPMENT AND A METHOD

National phase entry:
Publication Number WO/2023/099972
Publication Date 08.06.2023
International Application No. PCT/IB2022/057861
International Filing Date 23.08.2022
Title **
[English] AN IMMUNOFLUORESCENCE DETECTING EQUIPMENT AND A METHOD
[French] ÉQUIPEMENT ET PROCÉDÉ DE DÉTECTION D'IMMUNOFLUORESCENCE
Applicants **
L&T TECHNOLOGY SERVICES LIMITED DLF IT SEZ Park, 2nd Floor – Block 3, 1/124, Mount Poonamallee Road, Ramapuram, Chennai - 600 089, Tamil Nadu, India Chennai 600 089, IN
Inventors
GANJIKUNTA VENKATA, Vinay Kumar #26/1161, Rameswaram Street, Proddatur, Andhra Pradesh – 516360, India Proddatur 516360, IN
CHILUMULA, Saneeth Reddy #4-9-527/58, Road No.3, Rajarajeshwari Nagar Colony, Hayathnagar, Hyderabad, Telangana – 501505, India Hyderabad 501505, IN
RAGHAVAN, Vijaya #191, 2nd Cross, 2nd Stage, Brindavan Extension, Mysore, Karnataka – 570020, India Mysore 570020, IN
KUMAR, Vinod #320/E, 4th Cross, H G Road, K N Pura, Mysore, Karnataka – 570019, India Mysore 570019, IN
Priority Data
202141056012   02.12.2021   IN
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Quotation for National Phase entry

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China Filing972
EPO Filing, Examination7067
Japan Filing591
South Korea Filing575
USA Filing, Examination2710
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Total: 11915

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Abstract[English] The immunofluorescence equipment disclosed herein comprises a light emitting source 102, a lens tube 104, a microfluidic chip 202, a filter lens 108 and an avalanche photo diode (APD) based detector 106. The light emitting source 102 comprises at least an UV-LED. The UV-LED 102 is placed at a first circular face and a microfluidic chip 202 comprising at least one microfluidic channel 114 containing a biological sample with a biomarker is placed at a second circular face of the lens tube 104. The lens tube 104 comprises at least one biconvex lens 110. Light emitted from the UV-LED 102 is converged onto the at least one microfluidic channel 114 by the at least one biconvex lens 110 triggering the biomarker to emit immunofluorescent emissions at one or more wavelengths which are filtered by the filter lens 108 to allow detection of only required immunofluorescent emission.[French] L'invention concerne un équipement d'immunofluorescence comprenant une source d'émission de lumière (102), un tube de lentille (104), une puce microfluidique (202), une lentille filtrante (108) et un détecteur à base de photodiode à avalanche (APD) (106). La source d'émission de lumière (102) comprend au moins une LED UV. La LED UV (102) est placée au niveau d'une première face circulaire et une puce microfluidique (202) comprenant au moins un canal microfluidique (114) contenant un échantillon biologique avec un biomarqueur est placée au niveau d'une deuxième face circulaire du tube de lentille (104). Le tube de lentille (104) comprend au moins une lentille biconvexe (110). La lumière émise par la LED UV (102) est amenée à converger sur ledit canal microfluidique (114) par ladite lentille biconvexe (110) qui déclenche le biomarqueur pour émettre des émissions d'immunofluorescence à une ou plusieurs longueurs d'onde qui sont filtrées par la lentille filtrante (108) pour permettre la détection uniquement de l'émission d'immunofluorescence requise.
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