WO2022269543 - SPATIAL ANALYSIS OF A PLANAR BIOLOGICAL SAMPLE
National phase entry:
Publication Number
WO/2022/269543
Publication Date
29.12.2022
International Application No.
PCT/IB2022/055849
International Filing Date
23.06.2022
Title **
[English]
SPATIAL ANALYSIS OF A PLANAR BIOLOGICAL SAMPLE
[French]
ANALYSE SPATIALE D'UN ÉCHANTILLON BIOLOGIQUE PLAN
Applicants **
MOLECULENT AB
Neglinge Udde 4
13334 Saltsjöbaden, SE
Inventors
ERICSSON, Olof John
Neglinge Udde 4
133 34 Saltsjöbaden, SE
Priority Data
63/214,701
24.06.2021
US
63/257,456
19.10.2021
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
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| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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International Searching Authority |
EPO
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter II
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| * | |
| * | |
| Translation |
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Recalculate
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 3754 | |
| EPO | Filing, Examination | 46663 | |
| Japan | Filing | 591 | |
| South Korea | Filing | 607 | |
| USA | Filing, Examination | 17960 |
Total: 69575 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Provided herein, among other things, is method for analyzing a planar biological sample. In some embodiments, the method may comprise: contacting an oligonucleotide or a conjugate comprising the same with a planar biological sample under conditions by which the oligonucleotide or conjugate specifically binds to sites in or on the sample; performing one or more steps to release and/or extend the oligonucleotide in situ, to produce a reporter probe; transferring the reporter probe from the sample to a planar support that does not comprise an array of oligonucleotides, in a way that preserves the spatial relationship of the reporter probe in the sample; and detecting the reporter probe on the support.[French]
L'invention concerne, entre autres, un procédé d'analyse d'un échantillon biologique plan. Dans certains modes de réalisation, le procédé peut consister à : mettre en contact un oligonucléotide ou un conjugué comprenant celui-ci avec un échantillon biologique plan dans des conditions où l'oligonucléotide ou le conjugué se lie spécifiquement à des sites dans ou sur l'échantillon; réaliser une ou plusieurs étapes pour libérer et/ou étendre l'oligonucléotide in situ, pour produire une sonde rapporteur; transférer la sonde rapporteur de l'échantillon à un support plan qui ne comprend pas un réseau d'oligonucléotides, d'une manière qui préserve la relation spatiale de la sonde rapporteur dans l'échantillon; et détecter la sonde rapporteur sur le support.