WO2022249076 - METHODS TO PRODUCE DEFINED, SPHERICAL, BIO-DEGRADABLE MACROPOROUS MICROCARRIERS/HYDROGELS FOR CELLULAR AGRICULTURE

National phase entry:
Publication Number WO/2022/249076
Publication Date 01.12.2022
International Application No. PCT/IB2022/054873
International Filing Date 25.05.2022
Title **
[English] METHODS TO PRODUCE DEFINED, SPHERICAL, BIO-DEGRADABLE MACROPOROUS MICROCARRIERS/HYDROGELS FOR CELLULAR AGRICULTURE
[French] PROCÉDÉS DE PRODUCTION DE MICROSUPPORTS/HYDROGELS MACROPOREUX, SPHÉRIQUES ET BIODÉGRADABLES POUR L'AGRICULTURE CELLULAIRE
Applicants **
INNOCENT MEAT GMBH Landgut 9 18059 Papendorf, DE
Inventors
NONNENMACHER, Patrick Stampfmüllerstraße 32 18057 Rostock, DE
DRESCHER, Philipp Krischanweg 19 18069 Rostock, DE
Priority Data
63/192,700   25.05.2021   US
63/290,659   17.12.2021   US
17/751,943   24.05.2022   US
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Quotation for National Phase entry

Country StagesTotal
China Filing1975
EPO Filing, Examination11998
Japan Filing594
South Korea Filing640
USA Filing, Examination5910
MasterCard Visa

Total: 21117

The term for entry into the National Phase has expired. This quotation is for informational purposes only

Abstract[English] Biocompatible macroporous microcarriers, including microcarrier beads, microspheres, capsules, microsponges, hydrogels and other matrix forms, appropriate for use in a shaking flask or bioreactor to culture cells are described herein that can be used to create an edible structure for consumption or research investigation. Biocompatible, macroporous microcarriers can be dissolved or remain in the final product. Biocompatible macroporous microcarriers are formed by saccharides that are cross-linked via chemical induction with agitated cryo-gelation. Cross-linked macroporous, saccharide-microcarriers are coupled to adherence factors that enable cell binding. Finally, the cells are attached to the microcarrier for proliferation.[French] Microsupports macroporeux biocompatibles, comprenant des billes de microsupport, des microsphères, des micro-éponges, des hydrogels et d'autres formes de matrice, appropriés pour être utilisés dans un flacon à agitation ou un bioréacteur pour cultiver des cellules qui peuvent être utilisés pour créer une structure comestible pour la consommation ou la recherche. Les microsupports macroporeux biocompatibles peuvent être dissous ou rester dans le produit final. Les microsupports macroporeux biocompatibles sont formés par des saccharides réticulés par induction chimique avec cryogélation par agitation. Les microporteurs macroporeux réticulés à base de saccharides sont couplés à des facteurs d'adhérence pour permettre la fixation des cellules. Enfin, les cellules sont fixées au microsupport pour la prolifération.
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