WO2025003077 - MANUFACTURE OF POLYMERASE CHAIN AMPLIFICATION KITS OPTIMIZING AN AMPLIFICATION DENATURATION PHASE
National phase entry is expected:
Publication Number
WO/2025/003077
Publication Date
02.01.2025
International Application No.
PCT/EP2024/067690
International Filing Date
24.06.2024
Title **
[English]
MANUFACTURE OF POLYMERASE CHAIN AMPLIFICATION KITS OPTIMIZING AN AMPLIFICATION DENATURATION PHASE
[French]
FABRICATION DE KITS D'AMPLIFICATION EN CHAINE PAR POLYMERASE OPTIMISANT UNE PHASE DE DENATURATION DE L'AMPLIFICATION
Applicants **
BIOMÉRIEUX
69280 MARCY L'ETOILE, FR
Inventors
DRAZEK, Laurent
6 rue Louis et Auguste Lumière
38100 GRENOBLE, FR
PAILLIER, François
CircaGene DNA Genetic Testing
7 Bell Yard
WC2A2JR LONDRES, FR
VIDAL, Céline
17 rue des bleuets
38180 SEYSSINS, FR
Priority Data
FR2306813
28.06.2023
FR
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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| * | |
International Searching Authority |
EPO
* |
| Applicant's Legal Status |
Legal Entity
* |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Translation |
|
Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
** IP-Coster compiles data from publicly available sources. If this data includes your personal information, you can contact us to request its removal.
Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1379 | |
| EPO | Filing, Examination | 5482 | |
| Japan | Filing | 597 | |
| South Korea | Filing | 575 | |
| USA | Filing, Examination | 2710 |
Total: 10743 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Disclosed is a method for digitally simulating a polymerase chain amplification of at least one target, comprising the simulation of a plurality of successive cycles of the amplification, each cycle comprising, sequentially: a hybridization phase; an elongation phase; then a denaturation phase; wherein, for each cycle: the hybridization phase comprises: obtaining, for the cycle, an initial value of a vector of concentrations of a plurality of nucleic acid molecules in the form of single strands; calculating a matrix of concentrations of partial duplexes formed by the hybridization of the plurality of nucleic acid molecules in the form of single strands; the denaturation phase comprises: multiplying a vector of the concentration of elongated duplexes resulting from the application of the elongation phase to the concentrations of duplexes, by an elongated duplex denaturation tensor.[French]
Procédé de simulation numérique d'une amplification en chaîne par polymérase d'au moins une cible, comprenant la simulation d'une pluralité de cycles successifs de l'amplification, chaque cycle comprenant, de manière séquentielle : une phase d'hybridation; une phase d'élongation; puis une phase de dénaturation; dans lequel, pour chaque cycle : la phase d'hybridation comprend : l'obtention d'une valeur initiale pour le cycle d'un vecteur de concentrations d'une pluralité de molécules d'acide nucléique sous forme de simples brins; le calcul d'une matrice de concentrations de duplexes partiels formés par l'hybridation de la pluralité de molécules d'acide nucléique sous forme de simples brins; la phase de dénaturation comprend : la multiplication d'un vecteur de concentration de duplexes élongués, résultant de l'application de la phase d'élongation aux concentrations de duplexes, par un tenseur de dénaturation des duplexes élongués.