WO2025003074 - MANUFACTURE OF POLYMERASE CHAIN AMPLIFICATION KITS OPTIMIZING AN AMPLIFICATION HYBRIDIZATION PHASE
National phase entry is expected:
Publication Number
WO/2025/003074
Publication Date
02.01.2025
International Application No.
PCT/EP2024/067684
International Filing Date
24.06.2024
Title **
[English]
MANUFACTURE OF POLYMERASE CHAIN AMPLIFICATION KITS OPTIMIZING AN AMPLIFICATION HYBRIDIZATION PHASE
[French]
FABRICATION DE KITS D'AMPLIFICATION EN CHAINE PAR POLYMERASE OPTIMISANT UNE PHASE D'HYBRIDATION DE L'AMPLIFICATION
Applicants **
BIOMERIEUX
69280 MARCY L'ETOILE, FR
Inventors
DRAZEK, Laurent
6 rue Louis et Auguste Lumière
38100 GRENOBLE, FR
PAILLIER, François
CircaGene DNA Genetic Testing
7 Bell Yard
LONDRES WC2A 2JR, GB
VIDAL, Céline
17 rue des bleuets
38180 SEYSSINS, FR
Priority Data
FR2306808
28.06.2023
FR
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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International Searching Authority |
EPO
* |
| Applicant's Legal Status |
Legal Entity
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| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Translation |
|
Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1463 | |
| EPO | Filing, Examination | 6487 | |
| Japan | Filing | 597 | |
| South Korea | Filing | 575 | |
| USA | Filing, Examination | 2710 |
Total: 11832 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Disclosed is a method for digitally simulating a polymerase chain amplification of at least one target, comprising the simulation of a plurality of successive cycles of the amplification, each cycle comprising: a hybridization phase; an elongation phase; then a denaturation phase; wherein the hybridization phase comprises, for each cycle: obtaining, for the cycle, an initial value of a vector of concentrations of a plurality of nucleic acid molecules in the form of single strands; initializing a matrix of concentrations of duplexes formed by the hybridization of the plurality of nucleic acid molecules in the form of single strands; calculating the change in the matrix of concentrations during the hybridization phase by applying, to the matrix of concentrations, a matrix differential equation representing the kinetics of the duplex formation according to the concentrations of the plurality of nucleic acid molecules.[French]
Procédé de simulation numérique d'une amplification en chaîne par polymérase d'au moins une cible, comprenant la simulation d'une pluralité de cycles successifs de l'amplification, chaque cycle comprenant,: une phase d'hybridation; une phase d'élongation; puis une phase de dénaturation; dans lequel la phase d'hybridation comprend pour chaque cycle : l'obtention d'une valeur initiale pour le cycle d'un vecteur de concentrations d'une pluralité de molécules d'acide nucléique sous forme de simple brins; l'initialisation d'une matrice de concentrations de duplexes formés par l'hybridation de la pluralité de molécules d'acide nucléique sous forme de simples brins; le calcul, de l'évolution de ladite matrice de concentrations au cours de la phase d'hybridation, par l'application à la matrice de concentrations d'une équation différentielle matricielle représentant la cinétique de formation desdits duplexes en fonction des concentrations de la pluralité de molécules d'acide nucléique.