WO2025003074 - MANUFACTURE OF POLYMERASE CHAIN AMPLIFICATION KITS OPTIMIZING AN AMPLIFICATION HYBRIDIZATION PHASE
National phase entry:
Publication Number
WO/2025/003074
Publication Date
02.01.2025
International Application No.
PCT/EP2024/067684
International Filing Date
24.06.2024
Title **
[English]
MANUFACTURE OF POLYMERASE CHAIN AMPLIFICATION KITS OPTIMIZING AN AMPLIFICATION HYBRIDIZATION PHASE
[French]
FABRICATION DE KITS D'AMPLIFICATION EN CHAINE PAR POLYMERASE OPTIMISANT UNE PHASE D'HYBRIDATION DE L'AMPLIFICATION
Applicants **
BIOMERIEUX
Inventors
DRAZEK, Laurent
PAILLIER, François
VIDAL, Céline
Priority Data
FR2306808
28.06.2023
FR
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
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| Pages of Specification | * |
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2409 | |
| EPO | Filing, Examination, Granting | 10924 | |
| Japan | Filing, Examination, Granting | 2329 | |
| South Korea | Filing, Examination, Granting | 2304 | |
| USA | Filing, Examination, Granting | 4740 |
Total:
22,706
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
Disclosed is a method for digitally simulating a polymerase chain amplification of at least one target, comprising the simulation of a plurality of successive cycles of the amplification, each cycle comprising: a hybridization phase; an elongation phase; then a denaturation phase; wherein the hybridization phase comprises, for each cycle: obtaining, for the cycle, an initial value of a vector of concentrations of a plurality of nucleic acid molecules in the form of single strands; initializing a matrix of concentrations of duplexes formed by the hybridization of the plurality of nucleic acid molecules in the form of single strands; calculating the change in the matrix of concentrations during the hybridization phase by applying, to the matrix of concentrations, a matrix differential equation representing the kinetics of the duplex formation according to the concentrations of the plurality of nucleic acid molecules.[French]
Procédé de simulation numérique d'une amplification en chaîne par polymérase d'au moins une cible, comprenant la simulation d'une pluralité de cycles successifs de l'amplification, chaque cycle comprenant,: une phase d'hybridation; une phase d'élongation; puis une phase de dénaturation; dans lequel la phase d'hybridation comprend pour chaque cycle : l'obtention d'une valeur initiale pour le cycle d'un vecteur de concentrations d'une pluralité de molécules d'acide nucléique sous forme de simple brins; l'initialisation d'une matrice de concentrations de duplexes formés par l'hybridation de la pluralité de molécules d'acide nucléique sous forme de simples brins; le calcul, de l'évolution de ladite matrice de concentrations au cours de la phase d'hybridation, par l'application à la matrice de concentrations d'une équation différentielle matricielle représentant la cinétique de formation desdits duplexes en fonction des concentrations de la pluralité de molécules d'acide nucléique.