WO2024184407 - METHOD FOR IDENTIFYING TUMOR-SPECIFIC CELL SURFACE O-GLYCOPEPTIDES
National phase entry is expected:
Publication Number
WO/2024/184407
Publication Date
12.09.2024
International Application No.
PCT/EP2024/055880
International Filing Date
06.03.2024
Title **
[English]
METHOD FOR IDENTIFYING TUMOR-SPECIFIC CELL SURFACE O-GLYCOPEPTIDES
[French]
PROCÉDÉ D'IDENTIFICATION DE O-GLYCOPEPTIDES DE SURFACE CELLULAIRE SPÉCIFIQUES D'UNE TUMEUR
Applicants **
CALLEWAERT, Nico
Begijnhoflaan 15
9850 Deinze, BE
Inventors
WEYTS, Wannes
c/o VIB-UGent Center for Medical Biotechnology
Technologiepark-Zwijnaarde 75
9052 Gent, BE
DEVOS, Simon
c/o VIB-UGent Center for Medical Biotechnology
Technologiepark-Zwijnaarde 75
9052 Gent, BE
STAES, An
c/o VIB-UGent Center for Medical Biotechnology
Technologiepark-Zwijnaarde 75
9052 Gent, BE
DEMOL, Hans
c/o VIB-UGent Center for Medical Biotechnology
Technologiepark-Zwijnaarde 75
9052 Gent, BE
Priority Data
23160180.8
06.03.2023
EP
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
| * | |
| * | |
International Searching Authority |
EPO
* |
| Applicant's Legal Status |
Natural Person
* |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Translation |
|
Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1401 | |
| EPO | Filing, Examination | 4768 | |
| Japan | Filing | 535 | |
| South Korea | Filing | 576 | |
| USA | Filing, Examination | 2635 |
Total: 9915 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
The present invention relates to the field of glycoproteomics for identifying novel cell surface O-linked glycopeptide epitopes. The invention relates to methods for identifying O-linked glycopeptides, more specifically Tn- or SiaTn-antigen O-glycosylation sites on cell surface proteins, said method comprising a combined workflow of enzymatic treatments of cells or tissue samples to allow specific enrichment and labelling of Tn antigens, followed by mapping of the O-glycopeptides through LC-MS/MS. More specifically, the invention relates to a chemo-enzymatic method to produce labelled O-glycan-peptides from a plasmamembrane-protein extract sample, wherein Sialyltransferase (ST3Gal1/ST3Gal3) treatment is used to protect free galactose against a follow-on treatment with Galactose oxidase, wherein the oxidized glycans are simultaneously labelled. The invention further relates to a tandem mass spectrometry method using an enriched TMT-labelled (Sia)Tn-antigen glycopeptides in a dual fragmentation triggered approach to resolve the identity, localisation and quantification of the O-linked glycan peptides.[French]
La présente invention concerne le domaine de la glycoprotéomique en vue de l'identification de nouveaux épitopes glycopeptidiques à liaison O de surface cellulaire. L'invention concerne des procédés d'identification de glycopeptides à liaison O, plus précisément des sites de O-glycosylation d'antigènes Tn- ou SiaTn sur des protéines de la surface cellulaire, ledit procédé comprenant un flux de travail combiné associant des traitements enzymatiques d'échantillons de cellules ou de tissu pour permettre un enrichissement et un marquage spécifiques des antigènes Tn, suivis d'un mappage des O-glycopeptides par LC-MS/MS. Plus précisément, l'invention concerne un procédé chimio-enzymatique de production de O-peptidoglycanes marqués à partir d'un échantillon d'extrait de protéines membranaires plasmatiques, un traitement par la sialyltransférase (ST3Gal1/ST3Gal3) étant utilisé pour protéger le galactose libre contre un traitement de suivi par la galactose oxydase, les glycanes oxydés étant marqués simultanément. L'invention concerne en outre un procédé de spectrométrie de masse en tandem utilisant des glycopeptides d'antigène (Sia)Tn marqués par TMT enrichis dans le cadre d'une démarche déclenchée par une double fragmentation pour déterminer l'identité, la localisation et la quantification des peptidoglycanes à liaison O.