WO2023144061 - AN IMPROVED CELL CULTURE MEDIUM FOR CRUSTACEAN CELLS
National phase entry:
Publication Number
WO/2023/144061
Publication Date
03.08.2023
International Application No.
PCT/EP2023/051493
International Filing Date
23.01.2023
Title **
[English]
AN IMPROVED CELL CULTURE MEDIUM FOR CRUSTACEAN CELLS
[French]
MILIEU DE CULTURE CELLULAIRE AMÉLIORÉ POUR CELLULES DE CRUSTACÉS
Applicants **
UNIVERSITEIT GENT
Sint-Pietersnieuwstraat 25
9000 Gent, BE
Inventors
NAUWYNCK, Hans
Kerhoek 24
9930 Zomergem, BE
RAKHSHANINEJAD, Mostafa
Koningin Elisabethlaan 42
9820 Merelbeke, BE
Priority Data
22153353.2
26.01.2022
EP
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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International Searching Authority |
EPO
* |
| Applicant's Legal Status |
Legal Entity
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| * | |
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| Entry into National Phase under |
Chapter I
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| Translation |
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Recalculate
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1448 | |
| EPO | Filing, Examination | 5630 | |
| Japan | Filing | 595 | |
| South Korea | Filing | 574 | |
| USA | Filing, Examination | 2710 |
Total: 10957 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
The present invention relates to a cell culture medium which sustains the growth of cells obtained from a crustacean in vitro. Indeed, the present invention discloses a cell culture medium comprising a specific salt composition, in part responsible for the correct osmolality and pH, a specific amino acid and vitamin composition, energy sources such as glucose, and crustacean hemolymph. The present invention further discloses that said cell culture medium is capable to keep cells obtained from shrimp and lobster viable for long periods of time even after passaging the cells into subcultures and induces said cells to proliferate. The cultured cells are for example useful to perform physiological studies on certain genes (by genetic engineering) and to do research and diagnosis on crustacean pathogens.[French]
La présente invention concerne un milieu de culture cellulaire soutenant la croissance de cellules obtenues à partir d'un crustacé in vitro. En effet, la présente invention divulgue un milieu de culture cellulaire comprenant une composition saline spécifique, en partie responsable de l'osmolalité et du pH corrects, une composition spécifique d'acides aminés et de vitamines, des sources d'énergie telles que le glucose, et de l'hémolymphe de crustacé. La présente invention révèle en outre que ce milieu de culture cellulaire est capable de maintenir la viabilité des cellules provenant de crevettes et de homards pendant de longues périodes, même après le passage des cellules en sous-cultures, et qu'il induit la prolifération de ces cellules. Les cellules cultivées sont par exemple utiles pour réaliser des études physiologiques sur certains gènes (par génie génétique) et pour effectuer des recherches et des diagnostics sur les pathogènes des crustacés.