WO2023131727 - C-NUCLEOSIDE MONOPHOSPHATE SYNTHESIS
National phase entry:
Publication Number
WO/2023/131727
Publication Date
13.07.2023
International Application No.
PCT/EP2023/050489
International Filing Date
10.01.2023
Title **
[English]
C-NUCLEOSIDE MONOPHOSPHATE SYNTHESIS
[French]
SYNTHÈSE DE MONOPHOSPHATE DE C-NUCLÉOSIDE
Applicants **
UAB BIOMATTER DESIGNS
Žirmūnų g. 139A
LT-09120 Vilnius, LT
ENGINZYME AB
Tomtebodavägen 6
171 65 Solna, SE
Inventors
KOPLŪNAITĖ, Martyna
Antakalnio 102-39
LT-10201 Vilnius, LT
URBELIENĖ, Nina
L. Giros 4-41
LT-06325 Vilnius, LT
MEŠKYS, Rolandas
Rinktinės 19-72
LT-09202 Vilnius, LT
IKASALAITĖ, Diana
Balio Sruogos 10-1
LT-10220 Vilnius, LT
ZAKRYS, Linas
Laisvės pr. 99A-36
LT-06119 Vilnius, LT
KARPUS, Laurynas
Nemenčinės pl. 4C
LT-10109 Vilnius, LT
RUGGIERI, Federica
Skönviksvägen 307
122 66 Enskede, SE
GROBE, Sascha
Regnstigen 4
169 60 Solna, SE
Priority Data
2200248.9
10.01.2022
GB
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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International Searching Authority |
EPO
* |
| Applicant's Legal Status |
Legal Entity
* |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Translation |
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Recalculate
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1945 | |
| EPO | Filing, Examination | 12577 | |
| Japan | Filing | 590 | |
| South Korea | Filing | 575 | |
| USA | Filing, Examination | 9310 |
Total: 24997 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Provided herein is a one-pot method of synthesising a C-nucleoside-5'-monophosphate such as pseudouridine-5' -monophosphate, from an N-nucleoside using a multi-step enzymatic pathway. The method comprises reacting the N-nucleoside with a phosphate source and nucleoside phosphorylase to form a pentose-1-phosphate intermediate, reacting the pentose-1-phosphate intermediate with a phosphomutase to form pentose-5-phosphate intermediate, and reacting the pentose-5-phosphate intermediate with a nucleoside-5'-phosphate C -glycosidase to form the C-nucleoside-5'-monophosphate.[French]
La présente invention concerne un procédé de synthèse en une seule étape d'un C-nucléoside-5'-monophosphate, tel que la pseudouridine-5'-monophosphate, à partir d'un N-nucléoside en utilisant une voie enzymatique à plusieurs étapes. Le procédé consiste à faire réagir le N-nucléoside avec une source de phosphate et une nucléoside phosphorylase pour constituer un intermédiaire pentose-1-phosphate, à faire réagir l'intermédiaire pentose-1-phosphate avec une phosphomutase pour constituer l'intermédiaire pentose-5-phosphate, et à faire réagir l'intermédiaire pentose-5-phosphate avec une nucléoside-5'-phosphate C -glycosidase pour constituer le C-nucléoside-5'-monophosphate.