WO2023031205 - METHODS FOR ISOLATION OF LIPID-DISC COMPOSITIONS AND USES THEREOF
National phase entry:
Publication Number
WO/2023/031205
Publication Date
09.03.2023
International Application No.
PCT/EP2022/074095
International Filing Date
30.08.2022
Title **
[English]
METHODS FOR ISOLATION OF LIPID-DISC COMPOSITIONS AND USES THEREOF
[French]
PROCÉDÉS D'ISOLEMENT DE COMPOSITIONS DE DISQUES LIPIDIQUES ET LEURS UTILISATIONS
Applicants **
VIB VZW
Suzanne Tassierstraat 1
9052 Gent, BE
VRIJE UNIVERSITEIT BRUSSEL
Pleinlaan 2
1050 Brussel, BE
Inventors
REMAUT, Han
Kasteelstraat 10a
3370 Roosbeek, BE
NGUYEN, Van-Son
1040 Brussel, BE
JANSSENS, Arne
3040 Huldenberg, BE
Priority Data
21193811.3
30.08.2021
EP
22169108.2
20.04.2022
EP
Application details
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| Pages for Publication | * |
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International Searching Authority |
EPO
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 2005 | |
| EPO | Filing, Examination | 8418 | |
| Japan | Filing | 590 | |
| South Korea | Filing | 607 | |
| USA | Filing, Examination | 5760 |
Total: 17380 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
The invention relates to the field of bacterial membrane protein structures. More specifically, the invention relates to lipid nanodiscs compartmentalized by SlyB protein oligomers isolated from the outer membrane of Gram-negative bacteria. More specifically, the invention provides for a SlyB nanodisc structure wherein the SlyB-oligomer forms the membrane scaffold protein belt, which is surrounded by outer saccharolipid moieties anchored to the SlyB proteins, and which encloses a lipid bilayer nanodomain containing one or more phospholipid layers, wherein macromolecules such as (outer) membrane protein molecules may be captured and stabilized. More specifically, methods to produce and isolate chemically defined stable SlyB nanodisc particles are disclosed herein. Finally, the invention relates to the use of said SlyB nanodiscs as a self-adjuvanting vehicle, as part of an immunogenic composition, and provides for novel means for use in eliciting an immune response against macromolecules enclosed in said SlyB nanodiscs, or for use in a vaccine composition.[French]
L'invention relève du domaine des structures de protéines membranaires bactériennes. Plus particulièrement, l'invention concerne des nanodisques lipidiques compartimentés par des oligomères de protéine SlyB isolés à partir de la membrane externe de bactéries à Gram négatif. Plus particulièrement, l'invention concerne une structure de nanodisque SlyB, l'oligomère SlyB forme une bande de protéine d'échafaudage membranaire, qui est entourée par des fractions saccharidiques externes ancrées aux protéines SlyB, et qui renferme un nanodomaine bicouche lipidique contenant une ou plusieurs couches phospholipidiques, des macromolécules telles que des molécules de protéine membranaire (externe) peuvent être capturées et stabilisées. Plus particulièrement, l'invention concerne des procédés de production et d'isolement de particules de nanodisque SlyB stables chimiquement. Enfin, l'invention concerne l'utilisation desdits nanodisques de SlyB en tant que excipient auto-adjuvant, en tant que partie d'une composition immunogène, et de nouveaux moyens destinés à être utilisés pour déclencher une réponse immunitaire contre des macromolécules enfermées dans lesdits nanodisques SlyB, ou pour une utilisation dans une composition de vaccin.