WO2023012206 - METHOD FOR REDUCING ENDOTOXIN LEVELS IN NUCLEIC ACID PURIFICATION
National phase entry:
Publication Number
WO/2023/012206
Publication Date
09.02.2023
International Application No.
PCT/EP2022/071786
International Filing Date
03.08.2022
Title **
[English]
METHOD FOR REDUCING ENDOTOXIN LEVELS IN NUCLEIC ACID PURIFICATION
[French]
PROCÉDÉ DE RÉDUCTION DES TAUX D'ENDOTOXINES DANS LA PURIFICATION D'ACIDES NUCLÉIQUES
Applicants **
MERCK PATENT GMBH
Frankfurter Strasse 250
64293 Darmstadt, DE
Inventors
HEINEN-KREUZIG, Anja
c/o Merck KGaA
Frankfurter Strasse 250
64293 DARMSTADT, DE
KIESEWETTER, Andre
c/o Merck KGaA
Frankfurter Strasse 250
64293 DARMSTADT, DE
STEIN, Andreas
c/o Merck KGaA
Frankfurter Strasse 250
64293 DARMSTADT, DE
GUPTA, Akshat
c/o EMD Millipore Corporation
400 Summit Drive
BURLINGTON, Massachusetts 01803, US
Priority Data
63/229,666
05.08.2021
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
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| Number of Drawings | * |
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International Searching Authority |
EPO
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1433 | |
| EPO | Filing, Examination | 6863 | |
| Japan | Filing | 591 | |
| South Korea | Filing | 575 | |
| USA | Filing, Examination | 2710 |
Total: 12172 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
The present invention relates to a method for reducing endotoxin levels or removing endotoxins from nucleic acids. For this a non-ionic detergent selected from the group of alkylglycosides and secondary alcohol alkoxylates or mixtures thereof is added prior or during anion exchange chromatographic purification of the nucleic acids using a membrane or monolith-based sorbent.[French]
La présente invention concerne un procédé pour réduire les taux d'endotoxines ou éliminer les endotoxines d'acides nucléiques. Pour cela, un détergent non ionique choisi dans le groupe des alkylglycosides et des alcoxylates d'alcools secondaires ou des mélanges de ceux-ci est ajouté avant ou pendant la purification chromatographique par échange d'anions des acides nucléiques à l'aide d'une membrane ou d'un sorbant monolithique.