WO2024169609 - ENGINEERED LYSINE DECARBOXYLASES FOR THE PREPARATION OF 1, 5-DIAMINOPENTANE
National phase entry is expected:
Publication Number
WO/2024/169609
Publication Date
22.08.2024
International Application No.
PCT/CN2024/074797
International Filing Date
30.01.2024
Title **
[English]
ENGINEERED LYSINE DECARBOXYLASES FOR THE PREPARATION OF 1, 5-DIAMINOPENTANE
[French]
LYSINE DÉCARBOXYLASES MODIFIÉES POUR LA PRÉPARATION DE 1, 5-DIAMINOPENTANE
Applicants **
ENZYMASTER (NINGBO) BIO-ENGINEERING CO., LTD.
Inventors
CAI, Baoqin
DONG, Xicong
LIN, Shijia
WANG, Jiyong
ZHANG, Die
BOCOLA, Marco
CHEN, Cheng
CHEN, Haibin
Priority Data
202310180893.1
18.02.2023
CN
Application details
| Total Number of Claims/PCT | * |
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International Searching Authority |
CNIPA
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| * | |
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 1635 | |
| EPO | Filing, Examination, Granting | 11845 | |
| Japan | Filing, Examination, Granting | 2117 | |
| South Korea | Filing, Examination, Granting | 1908 | |
| USA | Filing, Examination, Granting | 6540 |
Total:
24,045
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Provided are engineered lysine decarboxylases that can be used to synthesize 1,5-diaminopentane under industrially relevant conditions. Also provided are polynucleotides encoding the engineered lysine decarboxylases, host cells capable of expressing the engineered lysine decarboxylases, and methods for preparing 1,5-diaminopentane using the engineered lysine decarboxylases. The engineered lysine decarboxylase is developed from a wild-type lysine decarboxylase through a process of directed evolution, and the engineered lysine decarboxylase has a better activity and/or stability and tolerates a high substrate concentration compared to other lysine decarboxylases for the preparation of 1,5-diaminopentane, and thus has a good prospect for industrial application.[French]
La présente invention concerne des lysines décarboxylases modifiées pouvant être utilisées pour synthétiser le 1,5-diaminopentane dans des conditions industrielles appropriées. Sont également présentés des polynucléotides codant pour les lysines décarboxylases modifiées, des cellules hôtes pouvant exprimer les lysines décarboxylases modifiées et des procédés de préparation du 1,5-diaminopentane par l'utilisation des lysines décarboxylases modifiées. La lysine décarboxylase modifiée est développée à partir d'une lysine décarboxylase de type sauvage selon un processus d'évolution dirigée, et la lysine décarboxylase modifiée présente une meilleure activité et/ou stabilité et tolère une concentration élevée de substrat par comparaison avec d'autres lysine décarboxylases pour la préparation de 1, 5-diaminopentane, et a donc de bonnes perspectives d'application industrielle.