WO2024169609 - ENGINEERED LYSINE DECARBOXYLASES FOR THE PREPARATION OF 1, 5-DIAMINOPENTANE

National phase entry is expected:
Publication Number WO/2024/169609
Publication Date 22.08.2024
International Application No. PCT/CN2024/074797
International Filing Date 30.01.2024
Title **
[English] ENGINEERED LYSINE DECARBOXYLASES FOR THE PREPARATION OF 1, 5-DIAMINOPENTANE
[French] LYSINE DÉCARBOXYLASES MODIFIÉES POUR LA PRÉPARATION DE 1, 5-DIAMINOPENTANE
Applicants **
ENZYMASTER (NINGBO) BIO-ENGINEERING CO., LTD. North Century Avenue, (6-21) - (6-27), Block 015 333 Ningbo, Zhejiang 315000, CN
Inventors
CAI, Baoqin 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
DONG, Xicong 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
LIN, Shijia 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
WANG, Jiyong 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
ZHANG, Die 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
BOCOLA, Marco 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
CHEN, Cheng 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
CHEN, Haibin 333 North Century Avenue, (6-21) - (6-27), Block 015 Ningbo, Zhejiang 315000, CN
Priority Data
202310180893.1   18.02.2023   CN
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Quotation for National Phase entry

Country StagesTotal
China Filing1031
EPO Filing, Examination6955
Japan Filing591
South Korea Filing574
USA Filing, Examination4510
MasterCard Visa

Total: 13,661

The term for entry into the National Phase has expired. This quotation is for informational purposes only

Abstract[English] Provided are engineered lysine decarboxylases that can be used to synthesize 1,5-diaminopentane under industrially relevant conditions. Also provided are polynucleotides encoding the engineered lysine decarboxylases, host cells capable of expressing the engineered lysine decarboxylases, and methods for preparing 1,5-diaminopentane using the engineered lysine decarboxylases. The engineered lysine decarboxylase is developed from a wild-type lysine decarboxylase through a process of directed evolution, and the engineered lysine decarboxylase has a better activity and/or stability and tolerates a high substrate concentration compared to other lysine decarboxylases for the preparation of 1,5-diaminopentane, and thus has a good prospect for industrial application.[French] La présente invention concerne des lysines décarboxylases modifiées pouvant être utilisées pour synthétiser le 1,5-diaminopentane dans des conditions industrielles appropriées. Sont également présentés des polynucléotides codant pour les lysines décarboxylases modifiées, des cellules hôtes pouvant exprimer les lysines décarboxylases modifiées et des procédés de préparation du 1,5-diaminopentane par l'utilisation des lysines décarboxylases modifiées. La lysine décarboxylase modifiée est développée à partir d'une lysine décarboxylase de type sauvage selon un processus d'évolution dirigée, et la lysine décarboxylase modifiée présente une meilleure activité et/ou stabilité et tolère une concentration élevée de substrat par comparaison avec d'autres lysine décarboxylases pour la préparation de 1, 5-diaminopentane, et a donc de bonnes perspectives d'application industrielle.