WO2024001593 - ENGINEERED KETOREDUCTASES FOR THE PREPARATION OF CHIRAL ALCOHOLS AND METHODS THEREOF
National phase entry is expected:
Publication Number
WO/2024/001593
Publication Date
04.01.2024
International Application No.
PCT/CN2023/094905
International Filing Date
17.05.2023
Title **
[English]
ENGINEERED KETOREDUCTASES FOR THE PREPARATION OF CHIRAL ALCOHOLS AND METHODS THEREOF
[French]
CÉTORÉDUCTASES MODIFIÉES POUR LA PRÉPARATION D'ALCOOLS CHIRAUX ET PROCÉDÉS ASSOCIÉS
Applicants **
ENZYMASTER (NINGBO) BIO-ENGINEERING CO., LTD.
North Century Avenue, (6-21) - (6-27), Block 015 333
Ningbo, Zhejiang 315000, CN
Inventors
CHEN, Haibin
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
LIANG, Yafang
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
ZHANG, Yingxin
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
XIA, Zekun
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
ZHOU, Ameng
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
BOCOLA, Marco
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
XU, Junpeng
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
LUO, Xiao
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
MAO, Jingxian
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
HONG, Ruimei
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
WU, Xinwei
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
SUN, Lei
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
DAI, Yixin
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
WANG, Songxue
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
PENG, Qinli
333 North Century Avenue, (6-21) - (6-27), Block 015
Ningbo, Zhejiang 315000, CN
Priority Data
202210753932.8
29.06.2022
CN
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
| * | |
| * | |
International Searching Authority |
CNIPA
* |
| Applicant's Legal Status |
Legal Entity
* |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Translation |
|
Recalculate
* The data is based on automatic recognition. Please verify and amend if necessary.
** IP-Coster compiles data from publicly available sources. If this data includes your personal information, you can contact us to request its removal.
Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1180 | |
| EPO | Filing, Examination | 6816 | |
| Japan | Filing | 602 | |
| South Korea | Filing | 576 | |
| USA | Filing, Examination | 6835 |
Total: 16009 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Provided is an engineered ketoreductase polypeptide capable of preparing chiral alcohols, especially for catalyzing the reduction of ethyl 4-chloroacetoacetate to produce ethyl (R) -4-chloro-3-hydroxybutyrate. The polypeptides has high catalytic activity, high stereoselectivity, better thermal stability and solvent tolerance; the reaction process using engineered ketoreductase polypeptides may have a substrate loading of up to 426 g/L and a conversion of up to 99%.[French]
La présente invention concerne un polypeptide de cétoréductase modifié, capable de préparer des alcools chiraux, en particulier de catalyser la réduction du 4-chloroacétate d'éthyle pour produire du (R) -4-chloro-3-hydroxybutyrate d'éthyle. Les polypeptides ont une activité catalytique élevée, une stéréosélectivité élevée, une meilleure stabilité thermique et une meilleure tolérance aux solvants; le processus de réaction utilisant des polypeptides de cétoréductase modifiés peut présenter une charge de substrat allant jusqu'à 426 g/L et un taux de conversion allant jusqu'à 99 %.