WO2023072170 - A METHOD FOR PROMOTING THE DIFFERENTIATION OF STEM CELLS INTO FUNCTIONALLY MATURE ISLET β CELLS AND USE THEREOF
National phase entry is expected:
Publication Number
WO/2023/072170
Publication Date
04.05.2023
International Application No.
PCT/CN2022/127805
International Filing Date
27.10.2022
Title **
[English]
A METHOD FOR PROMOTING THE DIFFERENTIATION OF STEM CELLS INTO FUNCTIONALLY MATURE ISLET β CELLS AND USE THEREOF
[French]
PROCÉDÉ POUR FAVORISER LA DIFFÉRENCIATION DES CELLULES SOUCHES EN CELLULES β D'ÎLOTS DE LANGERHANS FONCTIONNELLEMENT MATURES ET SON UTILISATION
Applicants **
SUNSHINE LAKE PHARMA CO., LTD.
Northern Industrial Area, Songshan Lake
Dongguan, Guangdong 523000, CN
Inventors
SONG, Yizhe
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
LI, Jingqiu
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
OU, Zhensheng
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
GUO, Leilei
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
TANG, Tingting
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
XING, Peiwen
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
LIU, Jing
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
ZHAO, Huifang
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
ZHANG, Shidu
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
YE, Qunrui
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
CHEN, Xiaofeng
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
LI, Wenjia
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
FENG, Shanshan
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
LI, Jing
Dongyangguang Hi-tech Park, Zhen An Road No. 368, Shang Sha, Chang An Town
Dongguan, Guangdong 523871, CN
Priority Data
202111264612.8
28.10.2021
CN
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
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| Pages for Publication | * |
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International Searching Authority |
CNIPA
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing | 1066 | |
| EPO | Filing, Examination | 6366 | |
| Japan | Filing | 590 | |
| South Korea | Filing | 574 | |
| USA | Filing, Examination | 3910 |
Total: 12506 USD
The term for entry into the National Phase has expired. This quotation is for informational purposes only
Abstract[English]
Provided is a method for promoting the differentiation of pluripotent stem cells into functionally mature islet β cells. The method comprises: coculturing endocrine progenitor cells with medium containing an M3 muscarinic acetylcholine receptor agonist, so as to facilitate the differentiation of endocrine progenitor cells into islet β cells; wherein, the endocrine progenitor cells are generated from primitive gut tube cells through in vitro differentiation. During differentiation process from primitive gut tube cells to endocrine progenitor cells, the cells are not cocultured with medium containing the M3 muscarinic acetylcholine receptor agonist.[French]
La présente invention concerne un procédé permettant de favoriser la différenciation de cellules souches pluripotentes en cellules β d'îlots de Langerhans fonctionnellement matures. Le procédé comprend la coculture de cellules progénitrices endocrines avec un milieu contenant un agoniste du récepteur muscarinique de l'acétylcholine M3, afin de faciliter la différenciation des cellules progénitrices endocrines en cellules β d'îlots de Langerhans ; les cellules progénitrices endocrines étant générées à partir de cellules primitives du tube digestif par différenciation in vitro. Pendant le processus de différenciation des cellules primitives du tube digestif en cellules progénitrices endocrines, les cellules ne sont pas cocultivées avec un milieu contenant l'agoniste du récepteur muscarinique de l'acétylcholine M3.