WO2025132472 - METHOD FOR DETECTING VIABLE MICROORGANISMS WHICH ARE POTENTIALLY PRESENT IN A SAMPLE OF CELLULAR PRODUCTS
National phase entry is expected:
Publication Number
WO/2025/132472
Publication Date
26.06.2025
International Application No.
PCT/EP2024/086952
International Filing Date
17.12.2024
Title **
[English]
METHOD FOR DETECTING VIABLE MICROORGANISMS WHICH ARE POTENTIALLY PRESENT IN A SAMPLE OF CELLULAR PRODUCTS
[French]
MÉTHODE POUR DÉTECTER DES MICROORGANISMES VIABLES POTENTIELLEMENT PRÉSENTS DANS UN ÉCHANTILLON DE PRODUITS CELLULAIRES
Applicants **
BIOMERIEUX
Inventors
DE LA FOATA, Corinne
GALLET-GORIUS, Emmanuelle
PALAZUELO, Marc
RAYMOND, Jean-Claude
Priority Data
EP23217648
18.12.2023
EP
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
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| Number of Multi-Dependent Claims | * |
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| Pages of Specification | * |
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| 译文 |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2128 | |
| EPO | Filing, Examination, Granting | 9298 | |
| Japan | Filing, Examination, Granting | 2216 | |
| South Korea | Filing, Examination, Granting | 2197 | |
| USA | Filing, Examination, Granting | 4740 |
Total:
20,579
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The present invention relates to a method for isolating viable microorganisms which are potentially present in a sample comprising 106 to 108 immune cells or progenitor cells thereof, the method comprising the following steps: - bringing the sample into contact with: • a lysing composition comprising o a lysis buffer comprising a non-ionic detergent at a concentration of between 0.004% and 0.050% of the reaction volume o and/or a lysis solution comprising a saponin at a concentration of between 0.03% and 4% of the reaction volume; • an endonuclease for digesting the nucleic acids released by the action of the lysing composition; • an endopeptidase acting at a pH of between 7 and 8; - separating the viable microorganisms which are potentially present in the sample by filtering the sample through a filter, the pores of which have a diameter of between 0.30 and 0.50 μm. The present invention also relates to the associated solid-phase cytometry detection method.[French]
La présente invention concerne un procédé d'isolement de microorganismes viables potentiellement présents dans un échantillon comprenant 106 à 108 cellules immunitaires ou cellules progénitrices de ces dernières, comprenant les étapes suivantes : - Mettre en contact l'échantillon avec • une composition lysante comprenant o un tampon de lyse comprenant un détergent non-ionique à une concentration comprise entre 0,004% et 0,050% du volume réactionnel o et/ou une solution de lyse comprenant une saponine à une concentration comprise entre 0,03% et 4% du volume réactionnel • une endonucléase permettant de digérer les acides nucléiques libérés par l'action de la composition lysante • une endopeptidase agissant à un pH compris entre 7 et 8, - Séparer les microorganismes viables potentiellement présents de l'échantillon par filtration de l'échantillon à travers un filtre dont le diamètre des pores est compris entre 0,30 et 0,50 µm. La présente invention concerne également la méthode associée de détection en cytométrie en phase solide.