WO2024009279 - AFFINITY CHROMATOGRAPHY USING AN ELUTION BUFFER COMPRISING GLYCINE AND ARGININE

National phase entry:
Publication Number WO/2024/009279
Publication Date 11.01.2024
International Application No. PCT/IB2023/057035
International Filing Date 07.07.2023
Title [English] AFFINITY CHROMATOGRAPHY USING AN ELUTION BUFFER COMPRISING GLYCINE AND ARGININE [French] CHROMATOGRAPHIE D'AFFINITÉ UTILISANT UN TAMPON D'ÉLUTION COMPRENANT DE LA GLYCINE ET DE L'ARGININE
Applicants ** TAKEDA PHARMACEUTICAL COMPANY LIMITED 1-1, Doshomachi 4-chome Chuo-ku, Osaka 541-0045, JP
Inventors ** LU, Wei c/o TAKEDA PHARMACEUTICAL COMPANY LIMITED 300 Shire Way Lexington, Massachusetts 02421, US
Priority Data 63/359,120  07.07.2022  US
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Country Stages Total
China Filing 1979
EPO Filing, Examination 11933
Japan Filing 574
South Korea Filing 576
USA Filing, Examination 7235
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Abstract [English] The present invention provides, among other things, methods of purifying a protein or polypeptide by affinity chromatography, for example, custom affinity chromatography, followed by downstream chromatographic steps, comprising eluting the recombinant protein from the affinity column using an elution buffer wherein the elution buffer comprises glycine and arginine at a concentration such that conductivity is no greater than the conductivity limit of the subsequent chromatographic resin. [French] La présente invention concerne, entre autres, des procédés de purification d'une protéine ou d'un polypeptide par chromatographie d'affinité, par exemple, une chromatographie d'affinité adaptée, suivie d'étapes chromatographiques en aval, comprenant l'élution de la protéine recombinante à partir de la colonne d'affinité à l'aide d'un tampon d'élution, le tampon d'élution comprenant de la glycine et de l'arginine à une concentration telle que la conductivité n'est pas supérieure à la limite de conductivité de la résine chromatographique ultérieure.