WO2023111966 - CAPILLARY ELECTROPHORESIS PURITY ANALYSIS OF COMPLEMENTARY STRAND NUCLEIC ACID MOLECULES
National phase entry:
Publication Number
WO/2023/111966
Publication Date
22.06.2023
International Application No.
PCT/IB2022/062337
International Filing Date
16.12.2022
Title **
[English]
CAPILLARY ELECTROPHORESIS PURITY ANALYSIS OF COMPLEMENTARY STRAND NUCLEIC ACID MOLECULES
[French]
ANALYSE DE PURETÉ D'ÉLECTROPHORÈSE CAPILLAIRE DE MOLÉCULES D'ACIDE NUCLÉIQUE DE BRIN COMPLÉMENTAIRE
Applicants **
DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventors
LI, Tingting
YOWANTO, Handy
JONES, Elliott
MOLLAH, Sahana
Priority Data
63/290,822
17.12.2021
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
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| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
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| Pages of Specification | * |
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| 译文 |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2396 | |
| EPO | Filing, Examination, Granting | 13957 | |
| Japan | Filing, Examination, Granting | 2494 | |
| South Korea | Filing, Examination, Granting | 2763 | |
| USA | Filing, Examination, Granting | 5940 |
Total:
27,550
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The presently described and claimed disclosure relates to method for characterizing nucleic acid purity comprising denaturing a nucleic acid sample, loading the nucleic acid sample onto a capillary electrophoresis (CE) capillary, wherein the CE capillary is filled with a buffer comprising a polymer matrix, applying a separation voltage to the CE capillary, wherein during the separation of the nucleic acids, the temperature of the CE capillary is increased, and detecting nucleic acids separated from the nucleic acid sample with a detector. Kits and instructions for use are also described.[French]
La présente invention concerne un procédé de caractérisation de la pureté des acides nucléiques comprenant la dénaturation d'un échantillon d'acide nucléique, le chargement de l'échantillon d'acide nucléique sur un capillaire d'électrophorèse (CE), le capillaire CE étant rempli d'un tampon comprenant une matrice polymère, l'application d'une tension de séparation au capillaire CE, la température du capillaire CE étant augmentée pendant la séparation des acides nucléiques, et la détection des acides nucléiques séparés de l'échantillon d'acide nucléique à l'aide d'un détecteur. L'invention concerne également des kits et des instructions d'utilisation.