WO2025027155 - METHOD AND SYSTEM FOR CHARACTERISING MICROORGANISMS BY DIGITAL HOLOGRAPHIC MICROSCOPY
National phase entry:
Publication Number
WO/2025/027155
Publication Date
06.02.2025
International Application No.
PCT/EP2024/071877
International Filing Date
01.08.2024
Title **
[English]
METHOD AND SYSTEM FOR CHARACTERISING MICROORGANISMS BY DIGITAL HOLOGRAPHIC MICROSCOPY
[French]
PROCÉDÉ ET SYSTÈME DE CARACTÉRISATION DE MICROORGANISMES PAR MICROSCOPIE HOLOGRAPHIQUE NUMÉRIQUE
Applicants **
BIOMÉRIEUX
BIOASTER
UNIVERSITE JEAN MONNET SAINT ETIENNE
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
Inventors
BRAULT, Dylan
FOURNIER, Corinne
THOMAS, Olivier
FAURE, Nicolas
Priority Data
23189164.9
02.08.2023
EP
23207095.3
31.10.2023
EP
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
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| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
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| Pages of Specification | * |
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| 译文 |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2105 | |
| EPO | Filing, Examination, Granting | 8396 | |
| Japan | Filing, Examination, Granting | 2093 | |
| South Korea | Filing, Examination, Granting | 1978 | |
| USA | Filing, Examination, Granting | 4740 |
Total:
19,312
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The invention relates to a method for characterising microorganisms which comprises: A. for each wavelength of a predetermined set of wavelengths comprising at least one wavelength, acquiring a holographic digital image, generating a focused image by computer means, each pixel of which image comprises an amplitude value and a phase value, and segmenting the focused image so as to extract portions corresponding to microorganisms; and B. characterising the microorganisms by computer means, according to the focused image portions. According to the invention, prior to the characterisation or prior to the segmentation or prior to the generation of the focused image, the method comprises correcting optical aberrations of the acquisition device by computer means, and the characterisation of the microorganisms comprises applying a digital model to these portions having as descriptors the amplitude and/or phase values of each wavelength of the predetermined set of wavelengths.[French]
Un procédé de caractérisation de microorganismes comprend A. pour chaque longueur d'onde d'un ensemble prédéterminé de longueurs d'onde d'au moins une longueur d'onde, l'acquisition d'une image numérique holographique la génération informatique d'une image focalisée dont chaque pixel comprend une valeur d'amplitude et une valeur de phase, la segmentation de l'image focalisée de manière à extraire des portions correspondant à des microorganismes; B. la caractérisation informatique des microorganismes en fonction desdites portions d'images focalisées, Selon l'invention, préalablement à la caractérisation ou préalablement à la segmentation ou préalablement à la génération de l'image focalisée, le procédé comporte la correction informatique d'aberrations optiques du dispositif d'acquisition et la caractérisation des microorganismes comporte l'application d'un modèle numérique auxdites portions ayant pour descripteurs les valeurs d'amplitude et/ou de phase de chaque longueur d'onde de l'ensemble prédéterminé de longueurs d'onde.