WO2024079161 - METAL-BINDING BACTERIAL PROTEIN FIBERS
National phase entry:
Publication Number
WO/2024/079161
Publication Date
18.04.2024
International Application No.
PCT/EP2023/078128
International Filing Date
11.10.2023
Title **
[English]
METAL-BINDING BACTERIAL PROTEIN FIBERS
[French]
FIBRES DE PROTÉINE BACTÉRIENNE LIANT UN MÉTAL
Applicants **
VIB VZW
VRIJE UNIVERSITEIT BRUSSEL
Inventors
REMAUT, Han
SLEUTEL, Mike
VAN MOLLE, Inge
SHERDRACK, Sylvia
Priority Data
22201184.3
12.10.2022
EP
Application details
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| 译文 |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2691 | |
| EPO | Filing, Examination, Granting | 11544 | |
| Japan | Filing, Examination, Granting | 2216 | |
| South Korea | Filing, Examination, Granting | 2197 | |
| USA | Filing, Examination, Granting | 9390 |
Total:
28,038
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The present invention relates to the field of bacterial protein engineering and protein fibers applicable as metal-binding bionanomaterials. More specifically, the present invention relates to engineered bacterial endospore appendage (Ena) proteins modified to contain metal-binding polypeptide (MBP) inserts providing for stable, flexible and robust protein assemblies with metal-binding activity. In particular, the invention relates to methods for designing Ena-fusion proteins capable of self-assembling into fibers, and for recombinant production of said self-assembling Ena-MBP fusion protein subunits, assemblies and fibers, ensuring a sustainable source of biological material for use in metal mineralization, metal sequestration, and metal-removal applications such as waste water treatment, water softening, or bioremediation.[French]
La présente invention relève du domaine de l'ingénierie des protéines bactériennes et des fibres protéiques pouvant être utilisées en tant que bionanomatériaux liant des métaux. Plus particulièrement, la présente invention concerne des protéines d'appendice d'endospore bactérien modifiées (Ena) modifiées pour contenir des inserts de polypeptide liant un métal (MBP) permettant d'obtenir des ensembles de protéines stables, flexibles et robustes ayant une activité de liaison métallique. En particulier, l'invention concerne des procédés de conception de protéines Ena-fusion capables d'auto-assemblage en fibres, et pour la production recombinante desdites sous-unités, ensembles et fibres de protéine de fusion Ena-MBP à auto-assemblage, assurant une source durable de matériau biologique destiné à être utilisé dans la minéralisation métallique, la séquestration de métal et des applications d'élimination de métal telles que le traitement des eaux usées, l'adoucissement de l'eau ou la bioremédiation.