WO2025250270 - POINT-OF-CARE DETECTION SYSTEM FOR AQUATIC MICROORGANISMS
National phase entry is expected:
Publication Number
WO/2025/250270
Publication Date
04.12.2025
International Application No.
PCT/US2025/025197
International Filing Date
17.04.2025
Title **
[English]
POINT-OF-CARE DETECTION SYSTEM FOR AQUATIC MICROORGANISMS
[French]
SYSTÈME DE DÉTECTION EN POINT DE SOINS POUR MICRO-ORGANISMES AQUATIQUES
Applicants **
GIANNASCA, Nicholas
Inventors
GIANNASCA, Nicholas
Priority Data
63/654,531
31.05.2024
US
Application details
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International Searching Authority |
USPTO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Natural Person
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2411 | |
| EPO | Filing, Examination, Granting | 10914 | |
| Japan | Filing, Examination, Granting | 2335 | |
| South Korea | Filing, Examination, Granting | 2431 | |
| USA | Filing, Examination, Granting | 4310 |
Total:
22,401
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Abstract[English]
A system and method are provided for detecting aquatic microorganisms. The system comprises a nucleic acid extraction step for extracting nucleic acid from aquatic samples, a nucleic acid amplification step for amplifying the extracted genomic material, and a nucleic acid detection step for detecting the presence of amplified nucleic acids, indicating the presence of microorganisms. The method and system are applicable to both marine and freshwater environments and can be used for point-of-care detection of pathogenic and/or beneficial aquatic microorganisms. A nucleic acid amplification component comprising a hand-held isothermal heater to amplify the extracted nucleic acid, and a nucleic acid detection component configured to detect amplified nucleic acid. The hand-held isothermal heater includes a main body, a sample port configured, to receive a sample tube, a viewing screen, a heat block, and an electronics compartment. The system enables rapid and efficient detection of pathogenic and beneficial aquatic microorganisms.[French]
Système et procédé pour détecter des micro-organismes aquatiques. Le système consiste à extraire l'acide nucléique des échantillons aquatiques, à amplifier le matériel génomique extrait et à détecter la présence d'acides nucléiques amplifiés, ce qui indique la présence de micro-organismes. La méthode et le système sont applicables à la fois aux environnements marins et d'eau douce et peuvent être utilisés pour la détection au point de soins de micro-organismes aquatiques pathogènes et/ou bénéfiques. La présente invention concerne égaleament un composant d'amplification d'acide nucléique comportant un dispositif de chauffage isotherme portatif destiné à amplifier l'acide nucléique extrait, et un composant de détection d'acide nucléique conçu pour détecter l'acide nucléique amplifié. Le dispositif de chauffage isotherme portatif comporte un corps principal, un orifice d'échantillonnage conçu pour accueillir un tube à échantillon, un écran de visualisation, un bloc chauffant et un compartiment électronique. Le système permet une détection rapide et efficace des micro-organismes aquatiques pathogènes et bénéfiques.