WO2025071426 - RAPID METHOD FOR ISOLATION OF MICROPLASTICS FROM MILK, YOGURT, SOUR CREAM AND BUTTER
National phase entry is expected:
Publication Number
WO/2025/071426
Publication Date
03.04.2025
International Application No.
PCT/RO2024/000010
International Filing Date
15.04.2024
Title **
[English]
RAPID METHOD FOR ISOLATION OF MICROPLASTICS FROM MILK, YOGURT, SOUR CREAM AND BUTTER
[French]
PROCÉDÉ RAPIDE D'ISOLEMENT DE MICROPLASTIQUES DE LAIT, DE YAOURT, DE CRÈME SURE ET DE BEURRE
Applicants **
UNIVERSITATEA "VALAHIA" DIN TÂRGOVIŞTE
Inventors
RĂDULESCU, Cristiana
DULAMĂ, Ioana Daniela
BĂNICĂ, Andreea Laura
BUCURICĂ, Ioan Alin
ŞTIRBESCU, Raluca-Maria
GORGHIU, Laura Monica
Priority Data
a202300528
26.09.2023
RO
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
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| Number of Pages with Drawings | * |
| Pages of Specification | * |
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| Number of Office Actions | * |
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| * | |
| * | |
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 1878 | |
| EPO | Filing, Examination, Granting | 8133 | |
| Japan | Filing, Examination, Granting | 1835 | |
| South Korea | Filing, Examination, Granting | 1369 | |
| USA | Filing, Examination, Granting | 4740 |
Total:
17,955
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The invention refers to a method for isolating microplastics (MPs) from milk and processed milk derivatives (yogurt, sour cream and butter) with applicability in the field of food safety. The method, according to the invention, consists of the following stages: A- pretreatment of the raw material (milk and processed milk products, with different fat content) with reagents of advanced purity, in the following mass ratios: milk:oxygenated water of 10:1, yogurt:NaOH:distilled water of 1:0,2:100, sour cream:sodium dodecyl sulfate:NaOH:distilled water of 1:0,1:0,12:100, butter:sodium dodecyl sulfate:NaOH:distilled water of 1:0.5:0.125:62.5, for digestion of the complex organic matrix, and homogenization by stirring at 150 rpm for 10 min; B-digestion proper by ultrasonication at 30°C, for 20 min; C-filtration under vacuum through filters with a porosity of 12...15 micrometers and maintaining the mixture in a water bath at 60°C until the filtration is complete; finally, the isolated MPs are quantified and characterized by complex analytical techniques.[French]
L'invention concerne un procédé d'isolement de microplastiques (MP) de lait et de dérivés de lait transformés (yaourt, crème sure et beurre) ayant une applicabilité dans le domaine de la sécurité alimentaire. Le procédé, selon l'invention, est constitué des étapes suivantes : A - prétraitement de la matière première (lait et produits laitiers transformés, à teneurs en matière grasse différentes) avec des réactifs de pureté avancée, selon les rapports massiques suivants : lait : eau oxygénée de 10 : 1, yaourt : NaOH : eau distillée de 1 : 0,2 : 100, crème sure : dodécylsulfate de sodium : NaOH : eau distillée de 1 : 0,1 : 0,12 : 100, beurre : dodécylsulfate de sodium : NaOH : eau distillée de 1 : 0,5 : 0,125 : 62,5, pour la digestion de la matrice organique complexe, et homogénéisation par agitation à 150 tr/min pendant 10 min; B - digestion proprement dite par ultrasons à 30 °C, pendant 20 min; C - filtration sous vide à travers des filtres de porosité 12... 15 micromètres et maintien du mélange dans un bain-marie à 60 °C jusqu'à achèvement de la filtration; enfin, les MP isolés sont quantifiés et caractérisés par des techniques analytiques complexes.