WO2025078978 - TRANSFECTION OF CELLS VIA REVERSIBLE PERMEABILIZATION
National phase entry is expected:
Publication Number
WO/2025/078978
Publication Date
17.04.2025
International Application No.
PCT/IB2024/059886
International Filing Date
09.10.2024
Title **
[English]
TRANSFECTION OF CELLS VIA REVERSIBLE PERMEABILIZATION
[French]
TRANSFECTION DE CELLULES PAR PERMÉABILISATION RÉVERSIBLE
Applicants **
AVECTAS LIMITED
Inventors
MARTIN, Darren
HACKETT, Emer
Priority Data
63/588,910
09.10.2023
US
63/620,207
12.01.2024
US
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
| * | |
| Number of Office Actions | * |
| * | |
International Searching Authority |
EPO
* |
| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
* |
| Type of Assignment |
The Standard Agent's Assignment
* |
| Applicant's Legal Status |
Legal Entity
* |
| * | |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Patent Delivery |
Send the Letters Patent by Courier
* |
| Translation |
|
* The data is based on automatic recognition. Please verify and amend if necessary.
** IP-Coster compiles data from publicly available sources. If this data includes your personal information, you can contact us to request its removal.
Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 3361 | |
| EPO | Filing, Examination, Granting | 15991 | |
| Japan | Filing, Examination, Granting | 2387 | |
| South Korea | Filing, Examination, Granting | 2516 | |
| USA | Filing, Examination, Granting | 7240 |
Total:
31,495
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner in an increased environmental temperature. A population of adherent cells is contacted with an aqueous solution including a payload and an alcohol at a concentration (v/v) greater than 2% in an environment having a temperature greater than 25°C. Also provided herein are non-viral methods for reprogramming blood cells to induced pluripotent stem cells (iPSCs). Related apparatus, systems, techniques, articles and compositions are also described.[French]
La présente invention concerne une plate-forme d'ingénierie cellulaire pour l'administration sans vecteur et/ou virale de composés et de compositions de charge utile/cargaison dans les cellules. La plateforme permet d'administrer les cellules rapidement et de manière simple dans un environnement à température élevée. Une population de cellules adhérentes est mise en contact avec une solution aqueuse comprenant une charge utile et un alcool à une concentration (v/v) supérieure à 2 % dans un environnement présentant une température supérieure à 25 °C. La présente invention concerne également des procédés non viraux de reprogrammation de cellules sanguines en cellules souches pluripotentes induites (iPSC). L’invention concerne également des appareils, des systèmes, des techniques, des compositions et des articles connexes.