WO2025046314 - IMPROVED HYBRIDIZATION ASSAY WITH STRINGENCY BUFFER DILUTION
National phase entry is expected:
Publication Number
WO/2025/046314
Publication Date
06.03.2025
International Application No.
PCT/IB2024/052463
International Filing Date
14.03.2024
Title **
[English]
IMPROVED HYBRIDIZATION ASSAY WITH STRINGENCY BUFFER DILUTION
[French]
DOSAGE D'HYBRIDATION AMÉLIORÉ AVEC DILUTION DE TAMPON DE STRINGENCE
Applicants **
FOSS ANALYTICAL A/S
Inventors
MATTHIESEN, Steen Hauge
PRITHWIRAJ, Kirtania
BERKICS, Balázs Viktor
CZÖMPÖLY, Tamás
Priority Data
PA202330176
30.08.2023
DK
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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| Number of Office Actions | * |
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International Searching Authority |
EPO
* |
| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
* |
| Type of Assignment |
The Standard Agent's Assignment
* |
| Applicant's Legal Status |
Legal Entity
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| * | |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Patent Delivery |
Send the Letters Patent by Courier
* |
| Translation |
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* The data is based on automatic recognition. Please verify and amend if necessary.
** IP-Coster compiles data from publicly available sources. If this data includes your personal information, you can contact us to request its removal.
Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 3124 | |
| EPO | Filing, Examination, Granting | 24330 | |
| Japan | Filing, Examination, Granting | 3105 | |
| South Korea | Filing, Examination, Granting | 3884 | |
| USA | Filing, Examination, Granting | 9740 |
Total:
44,183
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The present invention relates to a method for analysis of a sample for the presence of one or more target nucleic acids, the method comprising i) contacting the sample with one or more hybridization agents capable of hybridizing to said one or more target nucleic acids to obtain a mixture of the agent and the sample, ii) diluting the mixture obtained in i with a stringency buffer (such as a high-stringency buffer), in the presence of which specific hybridization between the one or more hybridization agents and their respective target nucleic acids is favored over non-specific hybridization events, iii) detecting, qualitatively or quantitatively, hybridization agent(s) hybridized to target nucleic acids in the mixture, which optionally is further diluted.[French]
La présente invention concerne un procédé d'analyse d'un échantillon pour détecter la présence d'un ou plusieurs acides nucléiques cibles, le procédé comportant les étapes suivantes : i) mise en contact de l'échantillon avec un ou plusieurs agents d'hybridation pouvant s'hybrider avec ledit ou plusieurs acides nucléiques cibles pour obtenir un mélange de l'agent et de l'échantillon ; ii) dilution du mélange obtenu en i avec un tampon de stringence (tel qu'un tampon de haute stringence), en présence duquel l'hybridation spécifique entre le ou les agents d'hybridation et leurs acides nucléiques cibles respectifs est favorisée par rapport aux événements d'hybridation non spécifiques ; iii) détection, qualitative ou quantitative, du ou des agents d'hybridation hybridés aux acides nucléiques cibles dans le mélange, qui est éventuellement encore dilué.