WO2025149785 - NUTRITIONAL COMPOSITION MADE FROM ISOLATION AND CHARACTERIZATION OF EXTRACELLULAR VESICLE SUBPOPULATIONS FROM XENOGENIC TISSUES AND ORGANS
National phase entry:
Publication Number
WO/2025/149785
Publication Date
17.07.2025
International Application No.
PCT/IB2024/052357
International Filing Date
12.03.2024
Title **
[English]
NUTRITIONAL COMPOSITION MADE FROM ISOLATION AND CHARACTERIZATION OF EXTRACELLULAR VESICLE SUBPOPULATIONS FROM XENOGENIC TISSUES AND ORGANS
[French]
COMPOSITION NUTRITIONNELLE PRÉPARÉE PAR ISOLEMENT ET CARACTÉRISATION DE SOUS-POPULATIONS DE VÉSICULES EXTRACELLULAIRES À PARTIR DE TISSUS ET D'ORGANES XÉNOGÉNIQUES
Applicants **
BADEN R&D LABORATORIES GMBH
Inventors
TAN, Kor Seng @ Chan Kok Seng
WONG, Bi Fah
SKUTELLA, Thomas
Priority Data
PI2024000310
11.01.2024
MY
Application details
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter II
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| Patent Delivery |
Send the Letters Patent by Courier
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| Translation |
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 1909 | |
| EPO | Filing, Examination, Granting | 8147 | |
| Japan | Filing, Examination, Granting | 2049 | |
| South Korea | Filing, Examination, Granting | 1803 | |
| USA | Filing, Examination, Granting | 4740 |
Total:
18,648
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Abstract[English]
The present invention provides a method for preparing a nutritional composition of xenogenic EVs from tissues/organs, comprising steps of collecting tissue/organ sample from fetal or adult mammal (101), keeping the collected sample in buffered saline in cold (102), mincing it into small pieces (103) and then mixing them with collagenase and deoxyribonuclease (104) followed by incubation and agitation (105), filtrating the incubated sample pieces for obtaining a filtrated liquid suspension containing extracellular fluid having EVs (106), differentially centrifuging the liquid suspension to remove remaining cells and tissue debris and obtain a supernatant (107), filtering the supernatant for collecting a crude fraction of large EVs (108) and centrifuging the supernatant for enriching it into a fraction of smaller EVs (109), characterized in that the separated EVs are fetal EV cargo or sinusoidal EVs directly isolated from the collected samples, wherein these EVs have regenerative potency, and the cells remain intact during EV isolation.[French]
La présente invention concerne un procédé de préparation d'une composition nutritionnelle de vésicules extracellulaires (EV) xénogéniques à partir de tissus/organes, comprenant les étapes de prélèvement d'un échantillon de tissu/organe à partir d'un mammifère fœtal ou adulte (101), maintien de l'échantillon prélevé dans une solution saline tamponnée au froid (102), hachage de celui-ci en petits morceaux (103) puis mélange de ceux-ci avec de la collagénase et de la désoxyribonucléase (104) suivi d'une incubation et d'une agitation (105), filtration des morceaux d'échantillon incubés pour obtenir une suspension liquide filtrée contenant du fluide extracellulaire contenant des EV (106), centrifugation différentielle de la suspension liquide pour éliminer les cellules et débris tissulaires résiduels et obtenir un surnageant (107), filtration du surnageant pour recueillir une fraction brute de grandes EV (108) et centrifugation du surnageant pour l'enrichir en une fraction d'EV plus petites (109), caractérisé en ce que les EV séparées sont une charge d'EV fœtales ou des EV sinusoïdales directement isolées à partir des échantillons collectés, ces EV ayant un pouvoir régénérateur, et les cellules restant intactes pendant l'isolement d'EV.