WO2025003119 - METHOD FOR DETECTING AND CONFIRMING SHIGA TOXIN-PRODUCING ESCHERICHIA COLI AND/OR ENTEROHAEMORRHAGIC E. COLI
National phase entry is expected:
Publication Number
WO/2025/003119
Publication Date
02.01.2025
International Application No.
PCT/EP2024/067785
International Filing Date
25.06.2024
Title **
[English]
METHOD FOR DETECTING AND CONFIRMING SHIGA TOXIN-PRODUCING ESCHERICHIA COLI AND/OR ENTEROHAEMORRHAGIC E. COLI
[French]
METHODE POUR LA DETECTION ET LA CONFIRMATION DES ESCHERICHIA COLI PRODUCTRICE DE SHIGATOXINE ET/OU D'UNE E. COLI ENTEROHEMORRAGIQUE
Applicants **
BIOMERIEUX
Inventors
CARRET, Justine
DOMINGOS, Céline
HAMON, Fabienne
JUNILLON, Thomas
MALLEN, Benoit
ROGER-CARDOSO, Tamara
Priority Data
23182506.8
29.06.2023
EP
Application details
| Total Number of Claims/PCT | * |
| Number of Independent Claims | * |
| Number of Priorities | * |
| Number of Multi-Dependent Claims | * |
| Number of Drawings | * |
| Pages for Publication | * |
| Number of Pages with Drawings | * |
| Pages of Specification | * |
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| Number of Office Actions | * |
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International Searching Authority |
EPO
* |
| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
* |
| Type of Assignment |
The Standard Agent's Assignment
* |
| Applicant's Legal Status |
Legal Entity
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| * | |
| * | |
| * | |
| * | |
| * | |
| Entry into National Phase under |
Chapter I
* |
| Patent Delivery |
Send the Letters Patent by Courier
* |
| Translation |
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* The data is based on automatic recognition. Please verify and amend if necessary.
** IP-Coster compiles data from publicly available sources. If this data includes your personal information, you can contact us to request its removal.
Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2090 | |
| EPO | Filing, Examination, Granting | 8573 | |
| Japan | Filing, Examination, Granting | 2227 | |
| South Korea | Filing, Examination, Granting | 2236 | |
| USA | Filing, Examination, Granting | 4740 |
Total:
19,866
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
(STEC) which may be present in a sample comprising enterobacteria, comprising the following steps: - performing lysis of the sample, enabling lysis of the STECs in order to obtain a solution comprising the nucleic acids thereof; - bringing the solution of nucleic acids into contact with primers, making it possible to amplify at least the stx1 and/or stx2 gene or gene fragment; - if at least one of the stx1 and/or stx2 genes or gene fragments is amplified, part of the sample is deposited on an agar reaction medium comprising ■ at least one toxin inducer, ■ at least one agglutinating conjugate formed by at least one binding partner specific to the STX1 protein and/or at least one binding partner specific to the STX2 protein, which binding partner(s) is (are) coupled to a nanoparticle; - detecting and confirming the presence of at least one STEC by the appearance of a halo on the agar around the STEC.[French]
productrice de shigatoxine (STEC) susceptible d'être présente dans un échantillon comprenant des entérobactéries, comprenant les étapes suivantes : - Effectuer une lyse de l'échantillon permettant la lyse des STEC afin d'obtenir une solution comprenant leurs acides nucléiques - Mettre en contact la solution d'acides nucléiques avec des amorces permettant d'amplifier au moins le gène ou le fragment de gène stx1 et/ou stx2 - Si au moins un des gènes ou fragments des gènes stxl et/ou stx2 est amplifié, une partie de l'échantillon est déposé sur un milieu réactionnel gélosé comprenant ■ au moins un inducteur de toxine, ■ au moins un conjugué agglutinant formé par au moins un partenaire de liaison spécifique de la protéine STX1 et/ou au moins un partenaire de liaison spécifique de la protéine STX2, couplé(s) à une nanoparticule. - Détecter et confirmer la présence d'au moins une STEC par l'apparition d'un halo sur la gélose autour de ladite STEC.