WO2023242189 - COLLAGEN VISUALIZATION ON MICROFLUIDIC DEVICE
National phase entry:
Publication Number
WO/2023/242189
Publication Date
21.12.2023
International Application No.
PCT/EP2023/065802
International Filing Date
13.06.2023
Title **
[English]
COLLAGEN VISUALIZATION ON MICROFLUIDIC DEVICE
[French]
VISUALISATION DE COLLAGÈNE SUR UN DISPOSITIF MICROFLUIDIQUE
Applicants **
ETH ZURICH
Inventors
MÜLLER, Ralph
QIN, Xiao-Hua
ZAUCHNER, Doris
Priority Data
22178671.8
13.06.2022
EP
22183705.7
07.07.2022
EP
Application details
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International Searching Authority |
EPO
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| Recordal of a Change of the Applicant's Name/Address |
Change of Applicant's Name and Address
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| Type of Assignment |
The Standard Agent's Assignment
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| Applicant's Legal Status |
Legal Entity
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| Entry into National Phase under |
Chapter I
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| Patent Delivery |
Send the Letters Patent by Courier
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Quotation for National Phase entry
| Country | Stages | Total | |
|---|---|---|---|
| China | Filing, Examination, Granting | 2269 | |
| EPO | Filing, Examination, Granting | 9327 | |
| Japan | Filing, Examination, Granting | 2207 | |
| South Korea | Filing, Examination, Granting | 2146 | |
| USA | Filing, Examination, Granting | 4740 |
Total:
20,689
The term for entry into the National Phase has expired. This quotation is for informational purposes only
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Abstract[English]
The invention relates to a method for generating an in-vitro cell culture model of cell development or cell differentiation, comprising a) providing a microfluidic chamber through which a stream of cell culture medium can be conducted; b) providing a plurality of primary cells embedded in a synthetic macroporous hydrogel, the macroporous hydrogel comprising polymer chains crosslinked by linker molecules amenable to cleavage by an extracellular endopeptidase, c) applying a flow of cell culture medium to said plurality of stem cells inside of the microfluidic chamber, thereby subjecting said cells to fluid shear stress in order to trigger functional maturation and collagen secretion. Another aspect of the invention relates to a microfluidic device, comprising a microfluidic chamber that comprises a macroporous hydrogel wherein cells are present. The macroporous hydrogel is composed of polymer chains crosslinked by linker molecules amenable to cleavage by an extracellular endopeptidase.[French]
L'invention concerne un procédé de génération d'un modèle de culture cellulaire in vitro de développement cellulaire ou de différenciation cellulaire, comprenant a) la fourniture d'une chambre microfluidique à travers laquelle un flux de milieu de culture cellulaire peut être conduit ; b) la fourniture d'une pluralité de cellules primaires incorporées dans un hydrogel macroporeux synthétique, l'hydrogel macroporeux comprenant des chaînes polymères réticulées par des molécules de liaison sensibles au clivage par une endopeptidase extracellulaire, c) l'application d'un flux de milieu de culture cellulaire à ladite pluralité de cellules souches à l'intérieur de la chambre microfluidique, soumettant ainsi lesdites cellules à une contrainte de cisaillement de fluide afin de déclencher une maturation fonctionnelle et une sécrétion de collagène. Un autre aspect de l'invention concerne un dispositif microfluidique, comprenant une chambre microfluidique qui comprend un hydrogel macroporeux dans lequel des cellules sont présentes. L'hydrogel macroporeux est composé de chaînes polymères réticulées par des molécules de liaison sensibles au clivage par une endopeptidase extracellulaire.